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Notes: The levels of NGF in culture medium of numerous human pancreatic cancer cell lines was determined using Promega's NGF E_max® ImmunoAssay System. NGF-induced pancreatic cancer cell growth seemed to be dependent on the expression levels and the balance of the TrkA and p75 receptors. (2334)

Notes: The levels of BDNF, NT-3 and NGF were examined in the cerebrospinal fluid of newborn rats (80 per sample) from postnatal day 1-21. NGF levels were consistently higher than the other neurotrophins ranging from 136pg/ml (day 21) to 607pg/ml (day 17) depending upon the day tested. NT-3 was the next most abundant ranging from approximately 40pg/ml (day 19) to 144pg/ml (day 7). BDNF was the least abundant ranging from 19.2pg/ml (day 5) to as high as 65pg/ml (day 13). NGF was determined with the NGF E_max^® ImmunoAssay System (NGF ELISA), NT-3 levels were determined with the NT-3 E_max^® ImmunoAssay System (NT-3 ELISA), and BDNF levels were determined with the BDNF E_max^® ImmunoAssay System (BDNF ELISA). (0167)

Notes: NT-4/5, NGF, and neurotrophin-3 (NT-3) was infused into cat primary visual cortex during a period of monocular deprivation. Cats were perfused transcardially with PBS, followed by 4% paraformaldehyde. The brain was embedded in gelatin-albumin in 50-80 µm sections. Sections were stained with Promega's Anti-Human NT-4/5, Anti-Human NT-3, or Anti-Human NGF Antibodies to determine the localization of these factors. (2369)

Notes: Human NGF was purified from term human placentas. The purified protein was recognized by the Anti-NGF mAb in a Western blot using the protocol in Neural Notes Vol.I Issue 1. (0798)

Notes: The NGF Emax^® ImmunoAssay System was used to determine the NGF content of mouse lavage and serum as well as the conditioned media of mononuclear cells. Allergic conditions showed increases in NGF. (1400)