Promega Corporation

CheckMate™ Mammalian Two-Hybrid System

Two-hybrid systems are extremely powerful methods for detecting protein:protein interactions in vivo. The basis of two-hybrid systems is the modular domains found in some transcription factors: a DNA-binding domain, which binds to a specific DNA sequence, and a transcriptional activation domain, which interacts with the basal transcriptional machinery. A transcriptional activation domain in association with a DNA-binding domain will promote the assembly of RNA polymerase II complexes at the TATA box and increase transcription. In the CheckMate™ Mammalian Two-Hybrid System the DNA-binding domain and the transcripti...

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CheckMate™ Mammalian Two-Hybrid System

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CheckMate™ Mammalian Two-Hybrid System

 Components

  • pBIND Vector

    E245A1 x 20μg
  • pACT Vector

    E246A1 x 20μg
  • pBIND-Id Control Vector

    E247A1 x 20μg
  • pACT-MyoD Control Vector

    E248A1 x 20μg
  • pG5luc Vector

    E249A2 x 20μg
1 system
- E2440 ¥ 5,542.00 Add to cart


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GenBank®/EMBL Accession Number

pACT Accession Number AF264723; pACT Vector sequence text file.
pBIND Accession Number AF264722; pBIND Vector sequence text file.
pG5luc, Accession Number AF264724; pG5luc Vector sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

pG5luc Vector.Figure 1. pG5luc Vector.
pACT Vector.Figure 2. pACT Vector.
pBIND Vector.Figure 3. pBIND Vector.
pACT-MyoD Control Vector.Figure 4. pACT-MyoD Control Vector.
pBIND-Id Control Vector.Figure 5. pBIND-Id Control Vector.

Use Restrictions

E2440 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

E2440 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

E2440 U.S. Pat. No. 5,670,356.

E2440 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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