Promega Corporation

FastBreak™ Cell Lysis Reagent

FastBreak™ Cell Lysis Reagent is designed for the efficient, gentle lysis of E. coli cultures without the need for centrifugation or mechanical cell disruption. The reagent is provided as a 10X concentrate and contains a proprietary nonionic detergent to facilitate lysis. Add the reagent directly to E. coli cultures. Following a brief incubation, the cells are disrupted, and the protein of interest is released. Recombinant proteins can be directly screened in the cell extract or purified by the addition of the appropriate affinity matrix such as the MagneHis™ Protein Purification System. This product is suitable f...

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FastBreak™ Cell Lysis Reagent, 10X

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FastBreak™ Cell Lysis Reagent, 10X

 Components

  • FastBreak™ Cell Lysis Reagent, 10X

    V857A1 x 15ml
15ml
- V8571 ¥ 547.00 Add to cart

FastBreak™ Cell Lysis Reagent, 10X

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FastBreak™ Cell Lysis Reagent, 10X

 Components

  • FastBreak™ Cell Lysis Reagent, 10X

    V857B1 x 60ml
60ml
- V8572 ¥ 1,691.00 Add to cart

FastBreak™ Cell Lysis Reagent, 10X

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FastBreak™ Cell Lysis Reagent, 10X

 Components

  • FastBreak™ Cell Lysis Reagent, 10X

    V857C1 x 100ml
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  • Helix Cabinet, Refrigerator

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet or a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs.


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100ml
- V8573 ¥ 2,266.00 Add to cart


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Storage Conditions

Store at 4–25°C.

For product intended use please see Patents & Disclaimers tab.

Manual purification of a 6X His-tagged fusion protein using FastBreak™ Cell Lysis Reagent and the MagneHis™ Protein Purification System.Figure 1. Manual purification of a 6X His-tagged fusion protein using FastBreak™ Cell Lysis Reagent and the MagneHis™ Protein Purification System.

Lane 1, bacterial cell lysate expressing 6X His-tagged firefly luciferase lysed by the addition of FastBreak™ Reagent; lane 2, postlysis cell supernatant demonstrating that the protein is solubilized; lane 3, flowthrough of cell lysate after the addition of MagneHis™ Ni-Particles; lane 4, eluted 6X His-tagged firefly luciferase; lane 5, Broad Range Protein Molecular Weight Markers (Cat.# V8491).

Use Restrictions

V8571, V8572, V8573 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V8571, V8572, V8573 Patent Pending.

V8571, V8572, V8573 This product is licensed for use under U.S. Pat. No. 6,174,704.

V8571, V8572, V8573 Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

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