Promega Corporation

PinPoint™ Xa Protein Purification System

The PinPoint™ Xa Protein Purification System is designed for the production and purification of fusion proteins that are biotinylated in vivo. The DNA coding for the protein of interest is cloned into a PinPoint™ Vector downstream of a sequence encoding a peptide that becomes biotinylated in vivo. Biotinylated fusion proteins are produced in E. coli and are affinity-purified using the SoftLink™ Soft Release Avidin Resin. This proprietary resin allows elution of the fusion protein under nondenaturing conditions. The PinPoint™ Vectors feature the encoded endoproteinase Factor Xa (pronounced "ten a") proteolytic site...

Expand to Read More »

  • Share
  • Print
  • Email
  • Prices valid for China customers only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

PinPoint™ Xa Protein Purification System

Close Window

PinPoint™ Xa Protein Purification System

 Components

  • SoftLink™ Soft Release Avidin Resin

    V201C1 x 3ml
  • PinPoint™ Xa-1 Vector

    V203A1 x 10μg
  • PinPoint™ Xa Control Vector

    V204A1 x 5μg
  • PinPoint™ Xa-2 Vector

    V205A1 x 10μg
  • PinPoint™ Xa-3 Vector

    V206A1 x 10μg
  • PinPoint™ Purification Column

    V556A1 x 1 each
  • Biotin

    V557A1 x 1ml
  • Streptavidin Alkaline Phosphatase

    V559A1 x 20μl
1 system
- V2020 ¥ 5,072.00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

PinPoint™ Xa-1 Vector

Close Window

PinPoint™ Xa-1 Vector

 Components

  • PinPoint™ Xa-1 Vector

    V203A1 x 10μg
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10μg
- V2031 ¥ 1,727.00 Add to cart

You Also May Be Interested In

GenBank®/EMBL Accession Number

PinPoint™ Xa-1, U47626; PinPoint™ Xa-2, U47627; PinPoint™ Xa-3, U47628.

Storage Conditions

Store the PinPoint™ Purification Column at room temperature. Store all remaining components at 4°C. The vectors may be stored at –20°C.

For product intended use please see Patents & Disclaimers tab.

PinPoint Xa-1 Vector.Figure 1. PinPoint™ Xa-1 Vector.

The PinPoint™ Xa-2 and Xa-3 Vector circle maps are identical to PinPoint™ Xa-1 Vector, except for one and two, respectively, additional adenosines at position 394.

Use Restrictions

V2020, V2031 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V2020 Exclusively licensed under U.S. Pat. No. 6,072,039.

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.