Promega Corporation

GoTaq® 1-Step RT-qPCR System
for Dye-Based DetectionPCR Satisfaction Guaranteed

Performing precise, accurate 1-step RT-qPCR analysis can be difficult when the target copy number is low or when PCR inhibitors are present. The GoTaq® 1-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol in a single tube. The BRYT Green® Fluorescent Dye and optimized buffer formulations improve data accuracy and sensitivity of low-level targets. The system is compatible with all real-time PCR instruments in both fast and standard cycling conditions.

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

GoTaq® 1-Step RT-qPCR System

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GoTaq® 1-Step RT-qPCR System

 Components

  • MgCl2

    A351B1 x 750μl
  • GoTaq® qPCR Master Mix, 2X

    A600A5 x 1ml
  • Nuclease-Free Water

    P119E2 x 13ml
  • CXR Reference Dye

    C541B1 x 200μl
  • GoScript™ RT Mix for 1-Step RT-qPCR

    M700A1 x 225μl
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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Request to add this product to your Helix »


200 × 50µl reactions
- A6020 ¥ 3,399.00 Add to cart


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PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

CXR Reference Dye

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CXR Reference Dye

 Components

  • CXR Reference Dye

    C541A1 x 100μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100μl
- C5411 ¥ 239.00 Add to cart

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Storage Conditions

Upon arrival, store all components at –30 to –10°C, protected from light. For immediate use, components may be stored at 2–8°C, protected from light, for up to 3 months.

For product intended use please see Patents & Disclaimers tab.

10302TA_600pxFigure 1. Sensitive quantification of GAPDH transcript, linear over a range of total RNA >7 orders of magnitude: 300ng–100fg of RNA sample per reaction with a detection of 10fg.

Triplicate standard reactions with 300ng, 100ng, 10ng, 1ng, 100pg, 10pg, 1pg, 100fg, NTC and six replicate reactions with 10fg of human total RNA were run using 200nM of each GAPDH transcript specific primer. Standard curve demonstrated 97% efficiency with R2 = 0.999. Three of six 10fg reactions amplified specifically and fit the standard curve, with an average Cq of 36, demonstrating linearity over range. NTC reactions were all blank.

Triplicate standard reactions with 300ng, 100ng, 10ng, 1ng, 100pg, 10pg, 1pg, 100fg, NTC and six replicate reactions with 10fg of human total RNA were run using 200nM of each GAPDH transcript specific primer. Standard curve demonstrated 97% efficiency with R2 = 0.999. Three of six 10fg reactions amplified specifically and fit the standard curve, with an average Cq of 36, demonstrating linearity over range. NTC reactions were all blank.

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Use Restrictions

A6020, C5411 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

A6020 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

A6020 NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 USA.

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