Promega Corporation

MethylEdge™ Bisulfite Conversion System

The MethylEdge™ Bisulfite Conversion System provides a rapid, efficient method to perform bisulfite conversion with minimal DNA fragmentation in less than two hours. The rapid protocol and complete conversion mean that you can produce completely converted DNA ready for your choice downstream assays with minimal preparation and hands-on time. Coupled with purification chemistries and flexible, robust amplification technologies for detection, the MethylEdge™ Bisulfite Conversion System delivers high-quality, intact converted DNA for your experiments.

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MethylEdge™ Bisulfite Conversion System

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MethylEdge™ Bisulfite Conversion System

 Components

  • Collection Tubes

    N124A1 x 50/pack
  • ME Spin Columns

    N125A1 x 50/pack
  • ME Elution Buffer

    N126A1 x 1ml
  • ME Desulphonation Buffer

    N127A1 x 10ml
  • ME Wash Buffer

    N128A1 x 6ml
  • ME Binding Buffer

    N129A1 x 30ml
  • ME Conversion Reagent

    N130A5 x 1.3ml
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  • Helix Cabinet

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.


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50 reactions
- N1301 ¥ 2,881.00 Add to cart


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Methylated Human Control

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Methylated Human Control

 Components

  • Methylated Human Control

    N123A1 x 5μg
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  • Helix Refrigerator

This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.


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5μg
- N1231 ¥ 2,594.00 Add to cart

Converted Methylated Human Control

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Converted Methylated Human Control

 Components

  • Converted Methylated Human Control

    N122A1 x 1μg
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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1μg
- N1221 ¥ 2,594.00 Add to cart

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Storage Conditions

Store the MethylEdge™ Bisulfite Conversion System at 22–25°C (room temperature). Store the Methylated Human Control at 2–10°C. Store the Converted Methylated Human Control at –30 to –10°C.

For product intended use please see Patents & Disclaimers tab.

Bisulfite conversion of unmethylated cytosines to uracil.Figure 1. Bisulfite conversion of unmethylated cytosines to uracil.

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Schematic diagram of the MethylEdge™ Bisulfite Conversion System protocol.Figure 2. Schematic diagram of the MethylEdge™ Bisulfite Conversion System protocol.

Convert DNA samples efficiently in less than 2 hours with minimal preprocessing steps.

Degree of DNA fragmentation after conversion.Figure 3. Degree of DNA fragmentation after conversion.

DNA was purified from whole blood using the ReliaPrep™ Blood gDNA Miniprep System (1) or Maxwell® 16 LEV Blood Kit (2) and was converted using the MethylEdge™ Bisulfite Conversion System (P) or a competing bisulfite conversion kit (Z or N). B = BenchTop 1kb DNA Ladder, D = nonconverted, purified DNA.

Use Restrictions

N1301, N1231, N1221 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

N1301, N1231, N1221 Use of Methylation-Specific PCR (MSP) is protected by U.S. Pat. Nos. 5,786,146, 6,017,704, 6,200,756 and 6,265,171. No license under these patents to use the MSP process is conveyed expressly or by implication to the purchaser by the purchase of this product.

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