QC Tests
Activity, endonuclease, DNase, RNase.
Source
Wheat germ.
Storage Buffer
50mM Tris-HCl (pH 7.9 at 25°C), 1mM EDTA, 1mM DTT, 50% (v/v) glycerol and 0.5M NaCl.
Storage Conditions
Store at –70°C.
Unit Definition
One unit is defined as the amount of enzyme required to convert 1μg of Form I to Form II pGEM®-9Zf(–) DNA in 30 minutes at 37°C. The reaction conditions are 50mM Tris-HCl (pH 7.5 at 25°C), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 1μg of supercoiled pGEM®-9Zf(–) DNA and 20% glycerol.
For product intended use please see Patents & Disclaimers tab.
Use Restrictions
M2851 For Research Use Only. Not for Use in Diagnostic Procedures.