The Erase-a-Base™ System is designed for the rapid construction of plasmid or M13 subclones containing progressive unidirectional deletions of any inserted DNA. The system is based on the procedure developed by Henikoff, in which exonuclease III (Exo III) is used to specifically digest insert DNA from a 5´ protruding or blunt end restriction site. The adjacent sequencing primer binding site is protected from digestion by a 4-base 3´ overhang restriction site or by an α-phosphorothioate-filled end.
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Erase-a-Base™ System (minus vectors & bacterial strain)
Erase-a-Base™ Control DNA
Exonuclease III 10X Buffer
S1 7.4X Buffer
S1 Stop Buffer
Klenow 1X Buffer
Ligase 10X Buffer
T4 DNA Ligase
DNA Polymerase I Large (Klenow) Fragment
This product can be added to a
This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Store at –20°C.
For product intended use please see Patents & Disclaimers tab.
E5750 For Research Use Only. Not for Use in Diagnostic Procedures.