Promega Corporation

Erase-a-Base™ System

The Erase-a-Base™ System is designed for the rapid construction of plasmid or M13 subclones containing progressive unidirectional deletions of any inserted DNA. The system is based on the procedure developed by Henikoff, in which exonuclease III (Exo III) is used to specifically digest insert DNA from a 5´ protruding or blunt end restriction site. The adjacent sequencing primer binding site is protected from digestion by a 4-base 3´ overhang restriction site or by an α-phosphorothioate-filled end.

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Erase-a-Base™ System (minus vectors & bacterial strain)

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Erase-a-Base™ System (minus vectors & bacterial strain)

 Components

  • Erase-a-Base™ Control DNA

    E179A1 x 10μg
  • S1 Nuclease

    E576A1 x 2,000u
  • Exonuclease III 10X Buffer

    E577A1 x 500μl
  • S1 7.4X Buffer

    E578A1 x 500μl
  • S1 Stop Buffer

    E579A1 x 1,000μl
  • Klenow 1X Buffer

    E580A1 x 1,000μl
  • dNTP Mix

    E581A1 x 500μl
  • Ligase 10X Buffer

    E582A2 x 1,000μl
  • DTT

    E583A1 x 250μl
  • PEG

    E584A2 x 1,000μl
  • T4 DNA Ligase

    M180A1 x 100u
  • Exonuclease III

    M181A2 x 5,000u
  • DNA Polymerase I Large (Klenow) Fragment

    M220A1 x 150u
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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1 system
- E5750 ¥ 8,074.00 Add to cart


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Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

E5750 For Research Use Only. Not for Use in Diagnostic Procedures.

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