Promega Corporation

ADP-Glo™ Kinase Assay

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ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

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ADP-Glo™ Kinase Assay

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ADP-Glo™ Kinase Assay

 Components

  • ADP-Glo™ Reagent

    V912A1 x 5ml
  • Kinase Detection Buffer

    V913A1 x 10ml
  • Kinase Detection Substrate

    V914A1 x 1 each
  • Ultra Pure ATP, 10mM

    V915A1 x 500μl
  • ADP, 10mM

    V916A1 x 500μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000 assays
- V9101 ¥ 6,375.00 Add to cart

ADP-Glo™ Kinase Assay

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ADP-Glo™ Kinase Assay

 Components

  • ADP-Glo™ Reagent

    V912B1 x 50ml
  • Kinase Detection Buffer

    V913B1 x 100ml
  • Kinase Detection Substrate

    V914B1 x 1 each
  • Ultra Pure ATP, 10mM

    V915B1 x 5ml
  • ADP, 10mM

    V916B1 x 5ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10,000 assays
- V9102 ¥ 33,590.00 Add to cart

ADP-Glo™ Kinase Assay

Close Window

ADP-Glo™ Kinase Assay

 Components

  • ADP-Glo™ Kinase Assay

    V910210 x 10,000 assays
100,000 assays
- V9103 ¥ 182,146.00 Add to cart
ADP-Glo™ Kinase Assay, Bulk Packaged
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ADP-Glo™ Kinase Assay, Bulk Packaged  Components

  • ADP-Glo™ Reagent

    V912B10 x 50ml
  • Kinase Detection Buffer

    V913B10 x 100ml
  • Kinase Detection Substrate

    V914B10 x 1 each
  • Ultra Pure ATP, 10mM

    V915B10 x 5ml
  • ADP, 10mM

    V916B10 x 5ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100,000 assays
- V9104 Please Enquire


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Storage Conditions

Store the system at –20°C. Before use, thaw all reagents completely at room temperature. Once thawed, components should be thoroughly mixed before use. Once prepared, the Kinase Detection Reagent (Kinase Detection Buffer + Substrate) should be divided into aliquots and stored at –20°C.

For product intended use please see Patents & Disclaimers tab.

Principle of the ADP-Glo Kinase Assay.Figure 1. Principle of the ADP-Glo™ Kinase Assay.

The assay is performed in two steps: 1) after the kinase or ATPase reaction, ADP-Glo™ Reagent is added to terminate the kinase reaction and deplete the remaining ATP, and 2) the Kinase Detection Reagent is added to convert ADP to ATP and allow the newly synthesized ATP to be measured using a luciferase/luciferin reaction. The light generated correlates to ADP present and kinase or ATPase activity.

Use Restrictions

V9101, V9102, V9103, V9104 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V9101, V9102, V9103, V9104 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

V9101, V9102, V9103, V9104 U.S. Pat. No. 7,700,310 and other patents and patents pending.

V9101, V9102, V9103, V9104 U.S. Pat. No. 7,741,067 and other patents and patents pending.

V9101, V9102, V9103, V9104 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

V9101, V9102, V9103, V9104 U.S. Pat. No. 8,183,007 and other patents pending.

V9101, V9102, V9103, V9104 Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

V9101, V9102, V9103, V9104 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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