Promega Corporation

GSH/GSSG-Glo™ Assay

The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. A change in GSH levels is important in the assessment of toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay provides a simple, rapid multiwell-plate format where stable luminescent signals are correlated with either the total GSH or the GSSG concentration of a sample directly in culture wells. Both total glutathione and GSSG determinations are based on the reaction where GSH-d...

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GSH/GSSG-Glo™ Assay

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GSH/GSSG-Glo™ Assay

 Components

  • Passive Lysis Buffer, 5X

    E194B1 x 1ml
  • DTT

    P117B1 x 250μl
  • Reconstitution Buffer with esterase

    V144A1 x 10ml
  • Glutathione Reaction Buffer

    V163A1 x 10ml
  • NEM, 25mM

    V164A1 x 50μl
  • Luciferin-NT

    V687A1 x 100μl
  • Glutathione S-Transferase

    V689B1 x 500μl
  • Glutathione, 5mM

    V690A1 x 100μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10ml
- V6611 ¥ 5,214.00 Add to cart

GSH/GSSG-Glo™ Assay

Close Window

GSH/GSSG-Glo™ Assay

 Components

  • Passive Lysis Buffer, 5X

    E194C1 x 5ml
  • DTT

    P117C1 x 1.25ml
  • Reconstitution Buffer with esterase

    V144B1 x 50ml
  • Glutathione Reaction Buffer

    V163B1 x 50ml
  • NEM, 25mM

    V164B1 x 250μl
  • Luciferin-NT

    V687B1 x 500μl
  • Glutathione S-Transferase

    V689B3 x 500μl
  • Glutathione, 5mM

    V690A2 x 100μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50ml
- V6612 ¥ 20,864.00 Add to cart


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Storage Conditions

Store at –20°C protected from light.

For product intended use please see Patents & Disclaimers tab.

GSH/GSSG ratio determination in different cell lines.Figure 1. GSH/GSSG ratio determination in different cell lines.

Cells were treated for 60 minutes with 40µM menadione or vehicle (0.1% DMSO). The protocol for adherent cells was performed and the ratio calculated as directed in TM344. Other cell lines tested: K562, CHO and Jurkat cells.

GSH-dependent conversion of a GSH probe, Luciferin-NT, to luciferin.Figure 2. GSH-dependent conversion of a GSH probe, Luciferin-NT, to luciferin by a glutathione S-transferase enzyme is coupled to a firefly luciferase reaction.

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Workflow for the GSH/GSSG-Glo Assay.Figure 3. Workflow for the GSH/GSSG-Glo™ Assay.
Limit of detection using reduced and oxidized glutathione.Figure 4. Limit of detection using reduced and oxidized glutathione.

GSH and GSSG standards were assayed in a 96-well plate using the GSH/GSSG-Glo™ System and a GloMax® Luminometer. The results were averaged and plotted on the graphs above. A linear trend line was generated and correlation coefficients calculated for the fit of the data to the trend line. Limits of detection were 2.5nM GSH and 0.5nM GSSG.

Patents - Disclaimers

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