Promega Corporation

BCIP/NBT Color Development Substrate (5-bromo-4-chloro-3-indolyl-phosphate/nitro blue...

BCIP/NBT Color Development Substrate (5-bromo-4-chloro-3-indolyl-phosphate/nitro blue tetrazolium)

BCIP (5-bromo-4-chloro-3-indolyl-phosphate) is used in conjunction with NBT (nitro blue tetrazolium) for the colorimetric detection of alkaline phosphatase activity. Each vial of BCIP is supplied with a vial of NBT.

Preparation of Substrates to Detect Alkaline Phosphatase: For every 5ml of alkaline phosphatase buffer (100mM Tris-HCl [pH 9.0], 150mM NaCl, 1mM MgCl2), add 33μl NBT and 16.5μl BCIP. Add the NBT first, mix, add the BCIP, and mix again. Use within 1 hour, and discard any unused solution.

Concentration: BCIP (50mg/ml) in 100% dimethylformamide; NBT (50mg/ml) in 70% dimethylformamide....

Expand to Read More »

  • Share
  • Print
  • Email
  • Prices valid for China customers only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

BCIP/NBT Color Development Substrate

Close Window

BCIP/NBT Color Development Substrate

 Components

  • NBT

    S380C1 x 2.5ml
  • BCIP

    S381C1 x 1.25ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1.25/2.5ml
- S3771 ¥ 585.00 Add to cart


You Also May Be Interested In

Storage Conditions

Store at either 4°C or –20°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

S3771 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.